Abstract
Our recent studies have demonstrated that microRNA miR‐1291 may play an important role in the regulation of tumorigenesis of pancreatic cancer cells. To understand the underlying mechanisms, we employed a LC/MS‐based metabolomics approach to determine the impact of miR‐1291 on pancreatic carcinoma cell metabolism. Our data showed that the miR‐1291 stably transfected and control PANC‐1 cells are readily segregated based upon their metabolomic profiles. A number of significantly altered cellular metabolites were identified, including 1‐methylnicotinamide (MNA), taurine, L‐carnitine, isobutyryl‐carnitine, isovaleryl‐carnitine and hydroxybutyryl‐carnitine. Among them, MNA was altered to the greatest extent, and the increase in cellular MNA was associated with a sharply elevated expression of nicotinamide N‐methyltransferase (NNMT). In addition, NNMT mRNA expression was inversely related to the size of PANC‐1 derived xenograft tumors. Our results suggest that MNA and NNMT are possible biomarkers in miR‐1291‐altered pancreatic carcinoma cell metabolism. The findings may help identify key metabolic pathways in PANC‐1 cells perturbed by miR‐1291 and understand their involvement in pancreatic tumorigenesis.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
