Abstract
The flavonoid dihydromyricetin (DMY) is the main component of Ampelopsis grossedentata (Hand-Mazz) W. T. Wang (AG), a daily beverage and folk medicine used in Southern China to treat jaundice hepatitis, cold fever, and sore throat. Recently, DMY and AG were shown to have a beneficial effect on lipid metabolism disorder. However, the mechanisms of how DMY and AG protect the liver during lipid metabolism disorder remain unclear. In this study, we first analyzed the chemical compounds of AG by HPLC-DAD-ESI-IT-TOF-MSn. Of the 31 compounds detected, 29 were identified based on previous results. Then, the effects of DMY and AG on high-fat diet hamster livers were studied and the metabolite levels and metabolic pathway activity of the liver were explored by 1H NMR metabolomics. Compared to the high-fat diet group, supplementation of AG and DMY attenuated the high-fat-induced increase in body weight, liver lipid deposition, serum triglycerides and total cholesterol levels, and normalized endogenous metabolite concentrations. PCA and PLS-DA score plots demonstrated that while the metabolic profiles of hamsters fed a high-fat diet supplemented with DMY or AG were both far from those of hamsters fed a normal diet or a high-fat diet alone, they were similar to each other. Our data suggest that the underlying mechanism of the protective effect of DMY and AG might be related to an attenuation of the deleterious effect of high-fat diet-induced hyperlipidemia on multiple metabolic pathways including amino acid metabolism, ketone body metabolism, energy metabolism, tricarboxylic acid cycle, and enhanced fatty acid oxidation.
Highlights
IntroductionToo its antioxidant [2] and anti-inflammatory effects [3], it can normalize blood lipid [4] and sugar [5] levels and attenuate liver injuries [6]
LVG hamsters were randomly divided into five groups with six animals in each group: a normal diet group (ND), a high-fat diet group (HFD), a high-fat diet group supplemented with 2 g/kg.d Ampelopsis grossedentata W. T. Wang (AG) (HFD + AG), a highfat diet group supplemented with 173 mg/kg.d DMY (HFD + DMY), and a high-fat diet group supplemented with 2.5 mg/kg.d simvastatin (HFD + SV). e water extract of AG and DMY was given from the start of the highfat diet to check their preventive effects, while SV was given two weeks after the lipid metabolism disorder model was set up
31 chemicals were detected and 29 flavonoids were identified in Ampelopsis grossedentata by HPLCDAD-ESI-IT-TOF-MSn
Summary
Too its antioxidant [2] and anti-inflammatory effects [3], it can normalize blood lipid [4] and sugar [5] levels and attenuate liver injuries [6]
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