Abstract
With increasing drug resistance of Pseudomonas aeruginosa, a new sterilization method is needed. Plasma has been shown to be an effective method of sterilization, but no researchers have studied the effect of plasma on bacterial metabolism. In this paper, we studied the emission spectrum, liquid phase active particles, and other physical and chemical properties of a portable plasma device. Pseudomonas aeruginosa were then treated with activated water generated by surface plasma discharge. Using gas chromatography time-of-flight mass spectrometry (GC-TOF-MS) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, we obtained the differential metabolite pathways. The results showed that, after plasma activated water treatment, the carbohydrate metabolism of the bacteria was inhibited and the metabolic processes of protein and amino acid decomposition were enhanced. Therefore, water activated by atmospheric-pressure cold plasma can significantly change bacterial metabolites, thus promoting bacterial death.
Highlights
Pseudomonas aeruginosa, is widely distributed in nature and is one of the most common bacteria in soil
Due to the high infection rate of Pseudomonas aeruginosa, it has a high level of drug resistance, and so a new sterilization method is needed [4]
Studies have demonstrated the bactericidal effect of atmospheric-pressure lowStudies have demonstrated the bactericidal effect of atmospheric-pressure low-temtemperature plasma, and it is generally believed that reactive nitrogen oxides are reperature it isofgenerally believedMeanwhile, that reactivesome nitrogen oxides areexplored responsible sponsibleplasma, for theand death bacteria [24,25,26,27]
Summary
Pseudomonas aeruginosa, is widely distributed in nature and is one of the most common bacteria in soil. According to the World Health Organization, Pseudomonas aeruginosa ranks second [1,2,3] among all bacterial infections. Due to the high infection rate of Pseudomonas aeruginosa, it has a high level of drug resistance, and so a new sterilization method is needed [4]. Atmospheric-pressure low-temperature plasma is a new technology where the temperature is similar to room temperature, so that plasma can be directly used in the treatment of organisms. Atmospheric-pressure cold plasma has been developed for biomedical applications, such as skin disease treatment, cancer treatment, beauty treatments for skin, dentistry, disinfection and sterilization applications [5,6,7,8,9,10,11]. The cost of plasma sterilization is much lower than that of traditional sterilization [14]
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