Abstract
Laxogenin C (LGC) is a natural spirostanol deriving from plant hormone which has shown growing regulation similar to those of brassinosteroids. In the present study, LGC showed a promoting effect on tomato seed germination and seedling growth in a dose-dependent manner. We applied LC-MS/MS to investigate metabolome variations in the tomato treated with LGC, which revealed 10 differential metabolites (DMs) related to KEGG metabolites, associated with low and high doses of LGC. Enrichment and pathway mapping based on the KEGG database indicated that LGC regulated expressions of 2-hydroxycinnamic acid and l-phenylalanine to interfere with phenylalanine metabolism and phenylpropanoids biosynthesis. The two pathways are closely related to plant growth and lignin formation. In our further phenotypic verification, LGC was confirmed to affect seedling lignification and related phenylpropanoids, trans-ferulic acid and l-phenylalanine levels. These findings provided a metabolomic aspect on the plant hormone derivates and revealed the affected metabolites. Elucidating their regulation mechanisms can contribute to the development of sustainable agriculture. Further studies on agrichemical development would provide eco-friendly and efficient regulators for plant growth control and quality improvement.
Highlights
Plant growth regulators (PGRs) are special chemicals that regulate the growth and development of plants at very low dosages [1], which play no phytotoxic roles
Laxogenin C (LGC) is a natural derivate of plant endogenous hormones and is widely used as an eco-friendly regulator for plant growth in agriculture
We applied LC-MS/MS to investigate the metabolome response to the regulator and revealed 10 differential metabolites (DMs) highly related to LGC regulation
Summary
Plant growth regulators (PGRs) are special chemicals that regulate (promote or inhibit) the growth and development of plants at very low dosages [1], which play no phytotoxic roles. There have been a variety of PGRs successfully used in agriculture, and new types of plant hormones, including brassinosteroids (BRs). They are attractive due to their involvement in many physiological procedures and abilities to promote seed germination [2] and plant growth [3,4], enable plants to resist the harsh living environment [5], and facilitate pesticide metabolism [6]. PGRs, especially those with new scaffolds, need deep insights into their functions and mechanisms where the yield and quality of vegetable products are increased and higher planting efficiency is achieved. Spirostanes share similarities in their physiological activity with BRs and have the potential to act as natural plant growth promoters. Steroidal glycosides of the spirostane series extracted from Nicotiana tabacum L
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
