Abstract
BackgroundPolysaccharides can alleviate obesity in mammals; however, studies on mechanism of this alleviation are limited. A few studies have indicated that polysaccharides improve obesity by regulating the metabolism of the body. Therefore, a metabolomics approach, consisting of high resolution nuclear magnetic resonance (NMR) spectroscopy and a multivariate statistical technique, was applied to explore the mechanism of the protective effects of lentinan and Flos Lonicera polysaccharides (LF) on high-fat diet (HFD) induced obesity.MethodsIn this study, rats were randomly divided into three groups: control diet (CD), HFD, and HFD supplemented with a mixture of lentinan and Flos Lonicera polysaccharide. Histopathological and clinical biochemical assessments were also conducted. A combination of a NMR metabolomics study and a multivariable statistical analysis method to distinguish urinary and fecal metabolites was applied.ResultsSignificant obesity symptoms appeared in HFD rats (for example, significant weight gain, epididymal adipose accumulation and lipid deposition in hepatocytes), which was attenuated in the LF group. Additionally, the HFD induced a reduction of choline, citrate, pyruvate and glycerol and increased the levels of trimethylamine oxide (TMAO) and taurine. Of note, these metabolic disorders were reversed by LF intervention mainly through pathways of energy metabolism, choline metabolism and gut microbiota metabolism.ConclusionsLF supplementation had a re-balancing effect on the disturbed metabolic pathways in the obese body. The results of this study validate the therapeutic effect of the compound polysaccharide--LF in obesity and described the biochemical and metabolic mechanisms involved.
Highlights
Polysaccharides can alleviate obesity in mammals; studies on mechanism of this alleviation are limited
The high-fat diet (HFD) supplemented with a mixture of lentinan and Flos Lonicera polysaccharide (LF, 0.675%, wt/wt) was prepared by Beijing HFK Bioscience Co., Ltd. (Beijing, China. 3Infinitus (China))
Effects of Lentinan and Flos Lonicera polysaccharides (LF) on high-fat diet induced obese rats Throughout the entire 12 weeks period, HFD feeding led to significant increases in body weight, epididymal adipose accumulation, and lipid deposition in hepatocytes compared with control diet feeding (Additional file 1: Figure S1, Fig. 1)
Summary
Polysaccharides can alleviate obesity in mammals; studies on mechanism of this alleviation are limited. A few studies have indicated that polysaccharides improve obesity by regulating the metabolism of the body. A metabolomics approach, consisting of high resolution nuclear magnetic resonance (NMR) spectroscopy and a multivariate statistical technique, was applied to explore the mechanism of the protective effects of lentinan and Flos Lonicera polysaccharides (LF) on high-fat diet (HFD) induced obesity. A compound polysaccharide, which is a mixture of lentinan and Flos Lonicera polysaccharides (LF), improved obesity and its related complications in our research studies (unpublished data). Despite the beneficial health effects of lentinan and FLP described above, few scientific studies have been conducted to elucidate the possible mechanisms by which this compound polysaccharide may exert its protective effects against obesity and its associated metabolic disorders. We aimed to evaluate the therapeutic effect of the compound polysaccharide lentinan and FLP on rat obesity
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