Abstract

Non-targeted metabolic profiling using high-resolution mass spectrometry (HRMS) is a standard approach for pathway identification despite technical limitations. To assess the performance of combining targeted quadrupole (QQQ) analysis with HRMS for in-depth pathway profiling. Serum of exercising patients with type 1 diabetes (T1D) was profiled using targeted and non-targeted assays. Non-targeted analysis yielded a broad unbiased metabolic profile, targeted analysis increased coverage of purine metabolism (twofold) and TCA cycle (three metabolites). Our screening strategy combined the benefits of the unbiased full-scan HRMS acquisition with the deeper insight into specific pathways by large-scale QQQ analysis.

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