Abstract
Myracrodruon urundeuva (“aroeira-do-sertao”) is a species threatened with extinction due to anthropogenic exploitation. Phytochemical analysis of bark, branch and leaf extracts revealed the presence of several compounds such as flavonoids, phenols, tannins, quercetin derivatives and anacardic acids. Dereplication methodology was performed to tentatively identify 50 compounds analyzed by ultra-performance liquid chromatography coupled with an electrospray ionization quadrupole time-of-flight mass spectrometry operating in MSE mode (UPLC-QTOF-MSE). The extracts exhibited anti-tumor effect in cancer cells HCT-116 (colorectal), SF-295 (glioblastoma), HL-60 (leukemia), and RAJI (leukemia). Also, these results correlate with the principal component analysis (PCA) data that identified three distinct groups indicating, efficiently, metabolic differences between organs of M. urundeuva. Through discriminatory analysis of the orthogonal partial least squares (OPLS-DA), the variable of importance in the projection (VIP) and S-Plot, we were able to determine 30 potential biomarkers. The fingerprint of hydroethanolic extracts was correlated with the cytotoxicity assay and demonstrated a significant difference in the composition of plant extract.
Highlights
Myracrodruon urundeuva Fr.All. (Anacardiaceae family), popularly known as “aroeira-do-sertão”, is a medicinal tree found in several regions of Brazil, especially in the caatinga.[1]
The extracts were analyzed by ultra-performance liquid chromatography (UPLC)-QTOF-MSE following the parameters described in “Chromatographic conditions” and “Mass spectrometry conditions” sub-sections only in the negative mode
From a simple and rapid extraction method, it was possible to trace the chemical profile of the three plant organs of M. urundeuva as leaf, branch and bark using the analytical technique UPLC-QTOF-MSE, which allowed the tentative identification of 50 compounds which covered several classes of compounds as flavonoids, flavanoids, hydrolysable tannins and anacardic acid
Summary
Myracrodruon urundeuva Fr.All. (Anacardiaceae family), popularly known as “aroeira-do-sertão”, is a medicinal tree found in several regions of Brazil, especially in the caatinga.[1]. After the combination of OPLS-DA, VIP and S-Plot it was possible to tentatively identify the possible biomarkers (Table 1) that may be associated with the highest cytotoxic activity presented in all biomarkers of leaf, bark and branch extracts.
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