Abstract
Background and Purpose: Magnolol, as the main active ingredient of Traditional Chinese Medicine, can significantly improve gastrointestinal motility disorders (GMD). In the present study, metabolomics was used to investigate the mechanism of magnolol improving L-arginine induced GMD in rats.Experimental Approach: SD rats were randomly divided into control group, model group and magnolol treated group. L-arginine was injected intraperitoneally in model and magnolol groups to induce GMD model. All intervention regimens were administered by oral gavage, once a day for five consecutive days. Relative gastric emptying rate and propulsive intestinal rate were measured. Metabolites in serum were analyzed based on UPLC-MS metabolomics technique.Results: Magnolol significantly promoted gastric emptying and small intestinal propulsion. Compared with the model group, the level of serotonin and L-tryptophan significantly reversed (P < 0.05) and 22 metabolites reversed in the magnolol group. According to MetPA database analysis, magnolol has mainly affected 10 major metabolic pathways which were related to each other, Tryptophan metabolism is the most critical metabolic pathway associated with gastrointestinal tract.Conclusion: These findings suggest that magnolol has a significantly promoting effect on L-arginine induced gastrointestinal motility disorder in rats, the mechanism is to reduce the production of nitric oxide to weaken the function of nitric oxide relaxing the gastrointestinal smooth muscle and increase the content of serotonin to promote gastrointestinal peristalsis and motility, secretion, absorption of nutrients.
Highlights
Gastrointestinal motility disorders (GMD) are a group of diseases, characterized by non-specific symptoms including nausea, vomiting, bloating, abdominal discomfort or pain, constipation or diarrhea
The results of gastric emptying and intestinal propulsion indicate that L-arginine had inhibited gastric emptying and intestinal propulsion, which indicated that the model of gastrointestinal motility disorder was successful
The results showed that magnolol could promote gastric emptying and small intestinal propulsion, it can effectively improve the gastrointestinal motility disorder in rats
Summary
Gastrointestinal motility disorders (GMD) are a group of diseases, characterized by non-specific symptoms including nausea, vomiting, bloating, abdominal discomfort or pain, constipation or diarrhea. It includes gastrointestinal dysfunction, screw weakening, and delays gastric emptying and intestinal propulsion (Beyder and Farrugia, 2012; Valentin et al, 2015). The most commonly used effective drugs for the treatment of GMD are cisapride and domperidone. Both of them were associated with adverse cardiovascular events (Chen et al, 2010; Arana et al, 2015). Metabolomics was used to investigate the mechanism of magnolol improving L-arginine induced GMD in rats. Metabolites in serum were analyzed based on UPLC-MS metabolomics technique
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