Metabolomic Study of Zuojin Pill in Relieving 1-Methyl-3-nitro-1-nitrosoguanidine-Induced Chronic Atrophic Gastritis.

Yuling Tong,Xu Zhao,Xia Liu,Min Wang,Ruisheng Li,Honghong Liu,Shizhang Wei,Manyi Jing,Yanling Zhao,Hongtao Song,K Arunachalam

doi:10.1155/2021/7004798

Abstract

The classic prescription Zuojin Pill (ZJP) shows a good therapeutic effect on chronic atrophic gastritis (CAG); it is of great significance to clarify its specific mechanism. Therefore, we explore the mechanism of ZJP on MNNG-induced CAG by integrating approaches. First of all, through the pathological changes of gastric tissue and the expression level of PGI and PGI/II in serum, the expression of inflammation-related factors was determined by RT-PCR to determine the efficacy. Then, UPLC-Q-TOF/MS was used for plasma and urine metabolomic analysis to screen the specific potential biomarkers and metabolic pathway of ZJP in ameliorating CAG and to explore its possible mechanism. ZJP significantly ameliorate the pathological injury of gastric tissue, increase levels of PGI and PGI/II, and reduce the expression level of proinflammatory factors. Through metabolomic analysis, 9 potential metabolic differences were identified and 6 related metabolic pathways were enriched. These findings indicate for the first time the potential mechanism of ZJP in improving CAG induced by MNNG and are of great significance to the clinical development and application of ZJP-related drugs.

Highlights

Gastric cancer (GC) is the fourth most common cancer and one of the leading causes of cancer-related death in the world [1]
Gastric mucosal atrophy is a key stage in the progression of GC, so paying attention to the prevention and treatment of Chronic atrophic gastritis (CAG) can effectively reduce the incidence of GC [3]. 1-Methyl-3nitro-1-nitrosoguanidine (MNNG) can cause base mutation on DNA and has strong carcinogenicity
Improve Serum Biochemical Indexes. e expression levels of IL-6, IL-1β, pepsinogen I (PGI), and pepsinogen II (PGII) in serum of rats were detected by Enzyme-linked immunosorbent assay (ELISA) kit. e results showed that the contents of PGI and PGI/PG in the MNNG group were significantly lower (P < 0.01 or P < 0.05), while the expression of IL-1β and IL-6 was significantly higher than the control group (P < 0.01 or P < 0.05) (Figure 2)

Summary

Introduction

Gastric cancer (GC) is the fourth most common cancer and one of the leading causes of cancer-related death in the world [1]. Gastric mucosal atrophy is a key stage in the progression of GC, so paying attention to the prevention and treatment of CAG can effectively reduce the incidence of GC [3]. E 2020 edition of Chinese Pharmacopoeia records that Coptis chinensis, processed with Tetradium ruticarpum (A.Juss.) T.G.Hartley juice, can relieve liver and stomach and stop vomiting. Tetradium ruticarpum (A.Juss.) T.G.Hartley has the effect of pungent, bitter, hot, antinausea, dispelling cold, and relieving pain. It is used for disharmony between the liver and stomach, vomiting, and swallowing acid. Recent research studies have shown that ZJP has a good therapeutic effect on gastric ulcer

Methods

Results

Conclusion