Abstract
In this study, a high-performance liquid chromatograph and an electrospray ionization (ESI) triple quadrupole mass spectrometry (TQ MS) detector were used to scan Oriental Beauty tea of different grades and prices. Principle component analysis (PCA) of the profiling data was performed for pattern recognition, clearly showing that the proposed MS profiling method was able to classify Oriental Beauty tea into different grades. The component mass ions primarily responsible for the separation were selected with high loading strength in the PCA for subsequent identification with tandem mass (MS/MS). Caffeine, citrate and salicylate were verified, whereas certain other compounds remained ambiguous. Regression analysis considering caffeine, citrate and salicylate showed a linear relationship between the prices of the Oriental Beauty tea with an adjusted R2 of 0.84. If all the selected marker ions (in addition to caffeine, citrate and salicylate) could have been identified and incorporated into regression analysis, a stronger relationship could have been confirmed. These results suggest that metabolomics can facilitate the determination of real markers in the quality control of Oriental Beauty tea, and may lead to the further application of metabolomics in other food quality controls.
Highlights
How to cite this paper: Chen, Y.-H., et al (2014) Metabolomic Studies Using High Performance Liquid Chromatography and Tandem Mass Spectrometry to Discover Quality Markers for Oriental Beauty (Dongfang Meiren) Tea
This study shows that using a single affordable triple quadrupole mass spectrometry (TQ mass spectrometry (MS)) detector for both metabolic profiling and component identification may require laborious works in annotation
Mass spectrometry is becoming more popular than nuclear magnetic resonance (NMR) in metabolomics because of its accuracy and data acquisition rate
Summary
How to cite this paper: Chen, Y.-H., et al (2014) Metabolomic Studies Using High Performance Liquid Chromatography and Tandem Mass Spectrometry to Discover Quality Markers for Oriental Beauty (Dongfang Meiren) Tea. Food and Nutrition Sciences, 5, 823-834. Metabolomics has become a promising research discipline that is synergistic with genomics and proteomics in system biology This technique can be used to study a living organism following the perturbation of diseases, chemicals, or gene defects [1]. Metabolomics includes the use of high-field proton nuclear magnetic resonance (NMR) or mass spectrometry (MS) techniques for the detection and identification of metabolites. Both of the techniques are effective and achieve a high throughput in profiling for pattern recognition. Chemical markers from metabolomics can serve as unbiased and complementary factors for judging quality
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