Metabolomic responses of Haliotis diversicolor to organotin compounds

Abstract

Organotin compounds, especially tributyltin (TBT) and triphenyltin (TPT), are a group of hazardous pollutants in marine environments. Haliotis diversicolor is an important marine model organism for environmental science. In this study, 1H NMR spectroscopy together with pattern recognition methods was used to investigate the responses of hepatopancreas and gill of Haliotis diversicolor to TBT and TPT exposure. It was found that obvious gender-, tissue- and compound-specific metabolomic alterations were induced after a 28-day exposure. TBT and TPT exposure not only caused the disturbance in energy metabolism and osmotic balance in hepatopancreas and gill tissues with different mechanisms, but also induced oxidative stresses. These metabolic alterations were highlighted in the accumulation of aspartate, uridine diphosphate-N-acetylglucosamine, uridine diphosphate glucose, guanosine and the depletion of leucine, isoleucine, valine, malonate, homarine, trigonelline in all exposure gills, as well as in the depletion of ATP, AMP, betaine in male exposure gills and pantothenate in male exposure hepatopancreases. The significant decreased aromatic amino acids (AAAs), lysine and glutamate in gills and increased betaine in hepatopancreases for TPT exposure together with increased glutamate and decreased betaine in gills and increased glutamate and glycine in hepatopancreases for TBT exposure demonstrated their specific metabolic characteristics. Among these characteristic metabolites, AAAs, lysine and glutamate in the gill as well as pantothenate in the hepatopancreas might be identified as potential biomarkers for TPT or TBT exposure in Haliotis diversicolor. The results provide a useful insight into the toxicological mechanisms of organotin compounds on Haliotis diversicolor.