Abstract
Endophytic fungi associated with medicinal plants are a potential source of novel chemistry and biology. Metabolomic tools were successfully employed to compare the metabolite fingerprints of solid and liquid culture extracts of endophyte Curvularia sp. isolated from the leaves of Terminalia laxiflora. Natural product databases were used to dereplicate metabolites in order to determine known compounds and the presence of new natural products. Multivariate analysis highlighted the putative metabolites responsible for the bioactivity of the fungal extract and its fractions on NF-κB and the myelogenous leukemia cell line K562. Metabolomic tools and dereplication studies using high-resolution electrospray ionization mass spectrometry directed the fractionation and isolation of the bioactive components from the fungal extracts. This resulted in the isolation of N-acetylphenylalanine (1: ) and two linear peptide congeners of 1: : dipeptide N-acetylphenylalanyl-L-phenylalanine (2: ) and tripeptide N-acetylphenylalanyl-L-phenylalanyl-L-leucine (3: ).
Highlights
In our search for new potential anticancer agents from endophytic fungi of Egyptian medicinal plants, Terminalia laxiflora (Combretaceae) was among the plants chosen for this study
These earlier reports on the genus Terminalia prompted us to look into the endophytes of T. laxiflora from which we isolated three endophytic fungi, Aspergillus aculeatus, Aspergillus oryzae and Curvularia sp
We focused on the isolation of the bioactive metabolites of a Curvularia extract against the leukemia cell line K562
Summary
In our search for new potential anticancer agents from endophytic fungi of Egyptian medicinal plants, Terminalia laxiflora (Combretaceae) was among the plants chosen for this study. We focused on the isolation of the bioactive metabolites of a Curvularia extract against the leukemia cell line K562. Putative metabolites responsible for the activity of the fungal extracts and fractions against leukemia cell lines were pinpointed by multivariate analysis.
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