Metabolomic fingerprint of cabbage resistance to Mamestra brassicae L. (Lepidoptera: Noctuidae).

Abstract

Plants defend themselves from insect feeding by activating specific metabolic pathways. We performed a metabolomic analysis to compare the metabolome reorganization that occurs in the leaves of two genotypes of cabbage (one partially resistant and one susceptible) when attacked by Mamestra brassicae caterpillars. The comparison of the metabolomic reorganization of both genotypes allowed us to identify 43 metabolites that are specifically associated with the insect feeding response in the resistant genotype. Of these, 19% are lipids or lipid-related compounds, most of which are modified fatty acids. These include glycosylated, glycerol-binding and oxidized fatty acids, the majority being associated with the oxylipin pathway. Some of the identified lipids are unlikely to be produced by plants and may be the result of biochemical reactions in the caterpillar oral secretions. A further 16% are phenylpropanoids. Interestingly, some phenylpropanoids were not present in the susceptible genotype, making them possible candidates for specific resistance-related compounds. Our results suggest that glucosinolates do not have a clear role in the resistance to M. brassicae feeding on cabbage. Using an untargeted metabolomics approach, we associated the regulation of metabolic pathways related to lipid signalling and phenylpropanoid compounds with the resistance to this pest. © 2022 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.