Abstract
DMBA is a potent carcinogenic widely used in experimental oncology. It has a great mutagenic capacity after being metabolically activated in the liver and induces an accelerated tumor growth. In order to evaluate metabolic alterations associated with DMBA administration the metabolic profile of liver was analysed by high resolution magic angle spinning NMR spectroscopy (HRMAS), a powerful method to study how cellular physiology is affected by xenobiotics.Fifteen male wistar rats were divided into two experimental groups: control and DMBA administration Liver samples of male Wistar rats were collected and snap frozen in liquid nitrogen and later analyzed by high rotation magic angle NMR spectroscopy (HRMAS) in a in a 14.1 Tesla Varian spectrometer.HRMAS spectral analysis followed by principal component statistical analysis demonstrated that glycogen, glucose and insaturated lipids were significantly reduced in the DMBA group, while aspartate was increased.The results of this study suggest that DMBA reduces hepatic insaturated lipid contents and glycogen plus glucose. Increased glycolytic fluxes are characteristic of tumors and could explain the reductions in carbohydrate stores. Increased aspartate and reduced insaturated fatty acids could be the result of an alteration in cytosolic redox, which also characterizes tumorigenic activity.
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