Metabolomic Approach for Characterization of Polyphenolic Compounds in Laminaria japonica, Undaria pinnatifida, Sargassum fusiforme and Ascophyllum nodosum.

Ping Shen,Hang Qi,Chenghang Sun,Chenxu Yu,Chunxu Zhang,Lei Qin,Yue Gu

doi:10.3390/foods10010192

Ping Shen, Hang Qi + Show 5 more

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https://doi.org/10.3390/foods10010192

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Abstract

Profiling of polyphenolics in four types of brown macroalgae, namely Laminaria japonica (L. japonica), Undaria pinnatifida (U. pinnatifida), Sargassum fusiforme (S. fusiforme), and Ascophyllum nodosum (A. nodosum), and their effect on oxidation resistance were investigated for the first time. Polyphenolic extracts from marine brown macroalgae were shown to effectively remove oxidants from cells and cellular systems. A. nodosum showed the highest antioxidant activity among evaluated brown macroalgae, showing a better scavenging effect on 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical and alleviating oxidative damage caused by hydrogen peroxide to human keratinocytes (HaCaT) cells. Through Q-Exactive HF-X mass spectrometry analysis, 12 polyphenolic compounds were preliminarily identified, including phlorotannins, phenolic acids, and flavonoids. Significant differences in content and variety of polyphenolics were found in evaluated brown macroalgae, which could be related to differences in antioxidant activity in vivo and in vitro. Moreover, the antioxidant activity might be related to the total phenolic content and the types of polyphenolics, especially phlorotannins. The findings presented in this study indicate that A. nodosum could be used as an important substitute for functional ingredients in foods and pharmaceutical preparations, as well as a raw material for phlorotannins research.

Highlights

In order to replace the previously used synthetic ingredients and potentially harmful ingredients, the industry is focusing its efforts and resources on the discovery and application of natural biologically active compounds [1,2]
In the last ten years, the marine ecosystem has been attracting the attention of researchers worldwide as it is a rich source of organisms possessing or producing substances with high biological activity
31.2 million tons, of which macroalgae aquaculture accounted for 96.5% [4]

Summary

Introduction

In order to replace the previously used synthetic ingredients and potentially harmful ingredients, the industry is focusing its efforts and resources on the discovery and application of natural biologically active compounds [1,2]. In the last ten years, the marine ecosystem has been attracting the attention of researchers worldwide as it is a rich source of organisms possessing or producing substances with high biological activity. Marine macroalgae (seaweeds) are a type of heterogeneous photosynthetic organisms different from land plants [3], having great potential industrial applications. The phytochemical composition of Laminaria, Sargassum, Ascophyllum, Ecklonia, Undaria, Himanthalia, and Dictyota have been widely studied [6]

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