Abstract
Globally, many crop production areas are threatened by drought and salinity. Potato (Solanum tuberosum L.) is susceptible to these challenging environmental conditions. In this study, an in vitro approach was employed to compare the tolerance of potato cultivars ‘BARI-401’ (red skin) and ‘Spunta’ (yellow skin). To simulate ionic and osmotic stress, MS media was supplemented with lithium chloride (LiCl 20 mM) and mannitol (150 mM). GC-MS and spectrophotometry techniques were used to determine metabolite accumulation. Other biochemical properties, such as total phenols concentration (TPC), total flavonoids concentration (TFC), antioxidant capacity (DPPH free radical scavenging capacity), polyphenol oxidase (PPO), and peroxidase (POD) activities, were also measured. The two cultivars respond differently to ionic and osmotic stress treatments, with Spunta accumulating more defensive metabolites in response, indicating a higher level of tolerance. While further investigation of the physiological and biochemical responses of these varieties to drought and salinity is required, the approach taken in this paper provides useful information prior to open field evaluation.
Highlights
Potato (Solanum tuberosum L.) is the leading tuber crop [1], with beneficial nutritional impact [2,3], but is very sensitive in terms of food security [4,5]
Antioxidant capacity measured by DPPH free radical scavenging capacity (FRSC) was higher (lower DPPH, half-maximal inhibitory concentration (IC50 )
In Spunta, Total phenols content (TPC) and total flavonoids concentration (TFC) were not affected at tested LiCl and mannitol concentrations (Figure 1a,b), antioxidant capacity measured by DPPH free radical scavenging capacity was higher in mannitol treatment compared to control and LiCl (Figure 1c)
Summary
Potato (Solanum tuberosum L.) is the leading tuber crop [1], with beneficial nutritional impact [2,3], but is very sensitive in terms of food security [4,5]. One of the major signatures of abiotic stress leading to yield reduction is the overproduction of reactive oxygen species (ROS) [14]. These ROS include superoxide anions (O2 − ), hydrogen peroxide (H2 O2 ), singlet oxygen (1 O2 ), and hydroxyl radicals (OH), which can be damaging and cause different physiological, molecular, and biochemical responses [15]. Production of secondary metabolites like phenols, flavonoids, tannins, saponins, and anthocyanin have been reported in different plant species under abiotic stresses [17].
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