Abstract

Several analytical methods may be used for metabolomic profiling of plants; however, 1H NMR spectra offers a wealth of information of metabolites compared to other methods. Decoupled NMR spectra (J-resolved) provide even more information since metabolites can be accurately integrated and it can exclude broad resonances from macromolecules and spin-spin coupling data. In addition, combinations of two dimensional NMR methods are quite helpful to identify the metabolites in plant extract.

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