Metabolomic alterations in invasive ductal carcinoma of breast: A comprehensive metabolomic study using tissue and serum samples.

Tushar H More,Manas K Santra,Srikanth Rapole,Joel Christie,Anupama Mane,Khushman Taunk,Koel Chaudhury,Sourav Roychoudhury

doi:10.18632/oncotarget.23626

Abstract

Invasive ductal carcinoma (IDC) is the most common type of breast cancer and the leading cause of breast cancer related mortality. In the present study, metabolomic profiles of 72 tissue samples and 146 serum samples were analysed using targeted liquid chromatography multiple reaction monitoring mass spectrometry (LC-MRM/MS) and untargeted gas chromatography mass spectrometry (GC-MS) approaches. Combination of univariate and multivariate statistical treatment identified significant alterations of 42 and 32 metabolites in tissue and serum samples of IDC, respectively when compared to control. Some of the metabolite changes from tissue were also reflected in serum, indicating a bi-directional interaction of metabolites in IDC. Additionally, 8 tissue metabolites and 9 serum metabolites showed progressive change from control to benign to IDC suggesting their possible role in malignant transformation. We have identified a panel of three metabolites viz. tryptophan, tyrosine, and creatine in tissue and serum, which could be useful in screening of IDC subjects from both control and benign. The metabolomic alterations in IDC showed perturbations in purine and pyrimidine metabolism, amino sugar metabolism, amino acid metabolism, fatty acid biosynthesis etc. Comprehensively, this study provides valuable insights into metabolic adaptations of IDC, which can help to identify diagnostic markers as well as potential therapeutic targets.

Highlights

Breast cancer is the most common malignancy observed in woman throughout the world with a prevalence of around 23% [1]
8 tissue metabolites and 9 serum metabolites showed progressive change from control to benign to Invasive ductal carcinoma (IDC) suggesting their possible role in malignant transformation
We have identified a panel of three metabolites viz. tryptophan, tyrosine, and creatine in tissue and serum, which could be useful in screening of IDC subjects from both control and benign

Summary

Introduction

Breast cancer is the most common malignancy observed in woman throughout the world with a prevalence of around 23% [1] It is the leading cause of cancer-related deaths in women with a mortality rate of about 14% [2]. Lack of specific diagnostic markers and unavailability of proper screening protocols at healthcare facilities significantly affects early diagnosis [11].

Methods

Results

Conclusion