Abstract
Metabolome-Wide Analysis of Stable Isotope Labeling-Is It Worth the Effort?
Highlights
Specialty section: This article was submitted to Systems Biology, a section of the journal Frontiers in Physiology
Stable isotope assisted metabolomics techniques have emerged as a valuable tool in systems biology for metabolic flux analysis and pathway discovery (Duckwall et al, 2013; Chokkathukalam et al, 2014; Niedenführ et al, 2015)
These enrichment patterns are analyzed by mass spectrometry (MS) or nuclear magnetic resonance (NMR) and are used to deduce metabolic fluxes (Truong et al, 2014; Young et al, 2014)
Summary
Specialty section: This article was submitted to Systems Biology, a section of the journal Frontiers in Physiology. Stable isotope assisted metabolomics techniques have emerged as a valuable tool in systems biology for metabolic flux analysis and pathway discovery (Duckwall et al, 2013; Chokkathukalam et al, 2014; Niedenführ et al, 2015). Stable isotope labeling analyses have been highly targeted, meaning that isotopic enrichment of only a small set of metabolites has been analyzed to deduce metabolic fluxes.
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