Metabolome profile variation in Azolla filiculoides exposed to Bisphenol A assists in the identification of stress-responsive metabolites

Abstract

This study attempted to explore the metabolome profile of Azolla filiculoides subjected to two different concentrations of BPA (1 and 30 mg L−1) in congruence with two different durations (3 and 9 days) of treatment. Bisphenol A (BPA) is a ubiquitously occurring environmental pollutant that imparts acute toxicity in aquatic plants. Therefore, studying the variations in the fern metabolome profile and identifying stress-responsive metabolites can help develop criteria for assessing the aquatic ecosystem. In recent times, metabolomics has drawn attention for its ability to detect biochemical processes and help link plant responses with environmental stresses. However, the studies concerning the metabolome profile of A. filiculoides exposed to environmental contaminants are limited. In the present study, the untargeted metabolomics study allowed the detection of a large array of metabolites, with 767 shared metabolites representing 41 crucial pathways. Exposure to 30 mg L−1 BPA seemingly disrupted the primary metabolism of the fern and induced a shift toward defense-related pathways. Additionally, BPA stress triggered the expression of metabolites like 3,4-dihydroxyphenylglycol, perillic acid, and perillaldehyde in BPA_L3 (1 mg L−1 for 3 days) and BPA_L9 (1 mg L−1 for 9 days) samples indicating protective mechanism of the plants. Conversely, the BPA_H3 (30 mg L−1 for 3 days) and BPA_H9 (30 mg L−1 for 9 days) samples expressed a distinct set of markers like luteolin, 3-hydroxyanthranilic acid, cinnamaldehyde, and l-DOPA indicating the onset of senescence and apoptosis related pathways can help in the health assessment of freshwater ecosystems and also appraisal of ecotoxicological risks imposed by BPA.