Abstract
Schizothorax o’connori (S. o’connori) is a representative tetraploid species in the subfamily Schizothoracinae and an important endemic fish in the Qinghai-Tibet Plateau. However, the domestication of S. o’connori remains challenging due to the lack of basic research. Here, we investigated the effects of artificial feeding on the oocytes and liver of S. o’connori by comparing the histological, metabolomic, and transcriptomic data. Histological results showed that the oocytes and liver of captive-reared S. o’connori had abnormal cell morphology. After comparison with the self-built database, a total of 233 metabolites were annotated. In oocytes, a total of 37 differentially accumulated metabolites (DAMs) were detected and two pathways were significantly enriched. There were obvious differences in the metabolites related to ovarian development, including pregnenolone and arachidonic acid. In liver, a total of 70 DAMs were detected and five pathways were significantly enriched. Based on the transcriptomic data, a total of 159 differentially expressed genes (DEGs) were significantly related with cell growth and death pathway in oocytes, while a total of 2841 DEGs were significantly related with 102 pathways in liver. Comparing the metabolomic and transcriptomic data showed that there were three common significant enrichment pathways in liver, including biosynthesis of unsaturated fatty acids, starch and sucrose metabolism, and fatty acid biosynthesis. These results showed that special attention should be given to the composition and intake of fatty acids during the artificial breeding of S. o’connori. In addition, many of metabolite-gene pairs were related to adenosine 5′-diphosphate, adenosine monophosphate, and pregnenolone. In summary, these data provide an overview of global metabolic and transcriptomic resources and broaden our understanding of captive-reared S. o’connori.
Highlights
Schizothorax o’connori (S. o’connori) is an important subfamily of endemic tetraploid fish in the main streams and tributaries of the Yarlung Tsangpo River (YLTR) on the Qinghai-Tibet Plateau (QTP) (Qi et al, 2012)
The results of Principal component analysis (PCA), partial least square discriminant analysis (PLS-DA), and OPLSDA showed that the wild and captive-reared groups were clearly different in both the liver and the oocytes, reflecting significant metabolic differences among the different groups (Table 1, Supplementary Figures S2, S3)
We report here the first histological, metabolomic, and transcriptomic analysis of liver and oocytes in wild and captive-reared S. o’connori
Summary
Schizothorax o’connori (S. o’connori) is an important subfamily of endemic tetraploid fish in the main streams and tributaries of the Yarlung Tsangpo River (YLTR) on the Qinghai-Tibet Plateau (QTP) (Qi et al, 2012) It is an excellent model organism for investigations of genome evolution and Quaternary climatic oscillations on the population structure of species endemic to the QTP (Guo. Metabolome and Transcriptome of Schizothorax o’connori et al, 2016; Xiao et al, 2020). Pregnenolone is synthesized from cholesterol which is synthesized in the liver (Mukherjee et al, 1991) Both liver and ovarian tissues play an important role in the reproductive process of various fish (Shirai et al, 2001; Hammond, 2011; Nagler et al, 2012; Qiao et al, 2019). Effects of artificial feeding on the oocyte and liver of S. o’connori remain poorly understood
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