Abstract
Rubus matsumuranus H. Lev. & Vaniot, a famous Siberian shrub of the Rosaceae family, is used in the folk medicine of nomads (Buryats, Yakuts, Soyots, and Mongols) as a remedy for the treatment of diseases of the respiratory and hepatobiliary systems. The lack of scientific information on R. matsumuranus leaves contributed to the investigation of the metabolomic profile and biological activity of this plant. In this study, metabolites of R. matsumuranus leaves in three stages (active growth, flowering, and fruiting) were characterised using high-performance liquid chromatography with photodiode array and electrospray ionisation triple quadrupole mass spectrometric detection (HPLC-PDA-ESI-tQ-MS). In total, 63 compounds were identified, including gallic acid derivatives, hydroxycinnamates, catechins, procyanidins, flavonols, and ellagitannins. Lambertianin C (57.11 mg/g of dry weight, DW), miquelianin (39.63 mg/g DW), and kaempferol-3-O-glucuronide (31.18 mg/g DW) were the major compounds in R. matsumuranus leaves. As a result of the HPLC-PDA-based assay to determine the antioxidant activity, it was revealed that lambertianin A, sanguiin H6, lambertianin C, and sanguiin H11 were effective scavengers of free radicals (2,2-diphenyl-1-picrylhydrazyl, DPPH•) and possessed Fe2+-chelating activity. After an investigation of the phenolic content in infusions and decoctions obtained by extraction with water at different temperatures, it was revealed that a hot infusion (80 °C) is a phenolic-rich preparation of R. matsumuranus leaves. Our research suggests that R. matsumuranus leaves are a rich source of phenolic compounds with high antioxidant properties and that this could be a prospective plant for new functional products.
Highlights
An increase in the range of medicines based on plant raw materials is one of the directions of development of the pharmaceutical industry [1]
The chromatographic profile of R. matsumuranus leaves was performed by highperformance liquid chromatography with photodiode array and electrospray ionisation triple quadrupole mass spectrometric detection (HPLC-PDA-ESI-tQ-MS)
The metabolites of R. matsumuranus leaves in three stages were studied for the first time
Summary
An increase in the range of medicines based on plant raw materials is one of the directions of development of the pharmaceutical industry [1]. Plant objects that are systematically close to the official ones and have a sufficient raw material base can be used. Such plants can be a possible source of functional food that has positive physiological benefits beyond their nutritional function [2]. The genus Rubus of the Rosaceous family is a potential source of functional products due to the variety of dietary fruits and leaf-based herbal teas [3,4,5,6]. The European Medicines Agency has approved the use of Rubus idaeus leaf infusions and extracts as herbal medicinal products based on their traditional uses [7]
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