Abstract
Oxygen uptake, product formation, and photon emission in the indole-3-acetic acid/peroxidase/O 2 system were inhibited by carbofuran and its metabolites, the 3-ketocarbofuran phenol and carbofuran phenol. These metabolites acted as competitive inhibitors and concomitantly were degraded. Photon emission, together with uv spectrophotometry and oxygen measurements, shows this to be a rapid and reproducible method to study insecticide interaction with the indole-3-acetic oxidase system in vitro. 3-Ketocarbofuran phenol was metabolized by peroxidase with excited-state production.
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