Metabolite Study of Cecal Ligation and Puncture-Induced Sepsis Rats Based on Macro Metabolomics

Abstract

Sepsis is a syndrome of a systemic inflammatory response. This study is to anlyze metabolites changes in sepsis. A rat model of sepsis was established by cecal ligation and puncture surgery. Then blood, ileal tissue and feces samples of rats were collected. The pathological changes of rat ileum and the levels of IL-1β, IL-6, HMGB-1, MCP-1, SOD and MDA were detected by HE staining and ELISA assay. FD-40 was detected to assess the integrity of the intestinal barrier. Fecal metabolites changes were analyzed by metabolomics based on UPLC-MS/MS system. Intestinal injuries were obvious in rat model of sepsis, the levels of IL-1β, IL-6, HMGB-1, MCP-1, MDA and FD-40 increased, while the content of SOD in the CLP group was decreased. For metabolomics analysis, the results showed profiles and changes in the sepsis rat fecal metabolite. Moreover, 15 different potential biomarker metabolites, mainly including Carbohydrates and Amino Acids. This study found that the key differential metabolites in the sepsis rats are carbohydrates and organic acids.