Abstract
Rice sheath blight is an important disease caused by Rhizoctonia solani. The resistant and susceptible rice lines (32R and 29S, respectively) showed different responses to R. solani infection in metabolite levels. The aim of this study was to characterize the metabolite levels in rice lines during R. solani infection using capillary electrophoresis equipped with time of flight mass spectrophotometry (CE/TOF-MS) in positive ion mode. Hundred metabolites were identified and classified into six clusters by hierarchical cluster using Mass Profiler Professional software. Changes in metabolite level at inoculated 32R and 29S were mapped on branches of tricarboxylic acid and glycolysis pathway. Volcano plot successfully filtered the metabolites based on fold change and p-value. The volcano plot result showed that 10 metabolites were up and down regulated in inoculated 32R relative to 29S. One metabolite, chlorogenic acid, showed a positive response in 32R. Meanwhile, pipecolic acid showed as the highest magnitude of fold change and p-value significance level in 29S. In addition, eight amino acids; glutamate, γ-aminobutyric acid, glycine, histidine, phenylalanine, serine, tryptophan, and tyrosine showed increase in 29S after R. solani inoculation.
Highlights
Rice sheath blight disease, caused by Rhizoctonia solani is a major disease affecting rice cultivation
CE/TOF-MS is an effective instrument for profiling metabolite in rice plant infected by sheath blight disease because the metabolic pathway responded to the infection were not distributed in a particular pathway in metabolic map, but randomly spread
Especially amino acids and phenols that involved in plant defence to R. solani infection can be detected and characterized by CE/TOF-MS
Summary
Rice sheath blight disease, caused by Rhizoctonia solani is a major disease affecting rice cultivation. Cultural practices and biological and chemical controls have been developed to ameliorate the problem (Nagarajkumar et al, 2004; Rodrigues et al, 2003; Slaton et al, 2003). The inheritance of rice resistance to sheath blight disease is mostly controlled by polygene. CN4-4-2 is susceptible hybrid Japonica rice from Chugoku 45 and Nipponbare (Wasano & Hirota, 1986). These resistant (32R) and susceptible (29S) rice lines were considered to be useful for the analysis of the mechanism of resistance to the sheath blight disease in rice
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