Metabolite Profiling Analysis of Conventional and Non-Conventional Extraction Methods on Secondary Metabolite from Peperomia pellucida (L.) Kunth Using UPLC-QToF-MS/MS System

Abstract

Objective : The purpose of this study was to observe the difference of extraction method (both conventional and non-conventional) based on metabolite profile using UPLC-QToF-MS/MS system from Peperomia pellucida (L.) Kunth. Methods: Dried samples were extracted using the conventional maceration method and the optimum ofionic liquid-based microwave-assisted extraction(IL-MAE) methods.Metabolite profiling was performed using UPLC-QToF-MS/MS system with some modifications adjusted to the instrument condition. The data was analyzed using Masslynx 4.1 software. Results: based on the results, there were differences on profiling metabolite from both conventional and non-conventional extraction methods that was extraction method using organic solvent and ionic liquid solvent ([BMIM]BF 4 at the optimum condition.The extract obtained using ILMAE method had a peak depth with a well-separated Rt (retention time) value ranging from 0.5 to 7.5 min which means that the extracted compound was from polar to nonpolar properties. The extract obtained using maceration method, the peak spread on a separate Rt ranges from 2.5 to 7.5 min. Also, both extracts obtained have different area under curve (AUC) values with amount total of 22285 (IL-MAE) and 12679 (maceration), respectively, and showed the IL-MAE twice as large as that of the maceration. Conclusion: based on the results, conventional and non-conventional extraction method showed differences in metabolite profiling based on Rt value and mass spectrum m/z of each peak. Key words: Peperomia pellucida (L) Kunth, Hyphenated system, Ionic liquid- based microwave-assisted extraction, Maceration, Metabolite profiling.