Abstract
Mitochondria from germinating castor bean endosperm were purified on linear sucrose-density gradients. It is shown that succinate and malate plus glutamate are oxidized rapidly; other substrates including malate (via malic enzyme) and external NADH are oxidized slowly. It is proposed that the oxidation of NADH produced during β-oxidation and the glyoxylate cycle occurs intramitochondrially via malate. Castor bean mitochondria are relatively impermeable to oxaloacetate and a malate-aspartate type shuttle is required. Metabolite transport and exchange properties support the operation of malatein (or succinatein)/2-oxoglutarateout and glutamatein/aspartateout shuttles. Not all the exchange systems were reversible. The results support proposed schemes for metabolite transfer between glyoxysomes and mitochondria of castor bean endosperm during germination.
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