Metabolite Fingerprinting Eleutherine palmifolia (L.) Merr. Using UPLC-QTOF-MS/MS

Roihatul Mutiah,Burhan Ma'Arif,Weka Sidha Bhagawan,Jauhar Maknun Septaza Rahmandika

doi:10.22146/mot.44883

Abstract

Eleutherine palmifolia (L.) Merr. (E. palmifolia) is an Indonesian native plant that has the potential to be developed into phytopharmaca. The differences in growth locations are thought to cause variation in the content of metabolite compounds which affect differences in pharmacological activity. This study aims to determine the profile of metabolites E. palmifolia bulb from several regions in Indonesia. The samples were collected from six different locations, namely East Java, Central Java, West Java, East Borneo, Central Borneo, and South Borneo. Sample extraction was carried out using Ultrasonic Assisted Extraction (UAE) method with 96% ethanol. The analysis of the content of metabolites was carried out using UPLC-QTOF-MS/MS with a stationary phase column C18 (Okta Decyl Silica), mobile phase mixture of formic acid /water 0.1/99.9 (v/v), and formic acid/acetonitrile 0,1/99,9 (v/v). The results of the analysis were interpreted using software Masslynx and continued with chemometric analysis using the method Principle Component Analysis (PCA). The results showed that there were differences in the content of the metabolite compounds in E. palmifolia bulb originating from six different regions.

Highlights

Metabolite fingerprinting is a fast and accurate method to determine the content of compounds contained in a plant extract using chromatographic techniques such as HighPerformance Thin Layer Chromatography (HPTLC), High-Performance Liquid Chromatography (HPLC), Gas chromatography (GC), and Mass spectrometry (MS) (Srivastava et al, 2010)
This study aims to determine the profile of the metabolites E. palmifolia bulb and determine whether there are differences in the composition of the metabolite compounds E. palmifolia bulb based on different growing conditions
Results of plant extraction Extraction of E. palmifolia bulb was carried out to obtain a concentrated extract of tuber bulb E. palmifolia

Summary

Introduction

Metabolite fingerprinting is a fast and accurate method to determine the content of compounds contained in a plant extract (metabolome) using chromatographic techniques such as HighPerformance Thin Layer Chromatography (HPTLC), High-Performance Liquid Chromatography (HPLC), Gas chromatography (GC), and Mass spectrometry (MS) (Srivastava et al, 2010). The metabolite profiling technique can be combined with profiling bioassays such as cytotoxic tests, antimicrobial tests, linked to the results of multivariate statistical analysis. Using these techniques, efforts to find new compounds can be done quickly and efficiently (Wolfendera et al, 2015). Eleutherine palmifolia (L.) Merr., is one of the native plants of Indonesia, especially the island of Borneo, which has the potential to be developed into phytopharmaca. E. palmifolia bulb contains secondary metabolites in the form of naphthoquinone and derivatives such as elecanacine, eleutherine, eleutherol, eleuthernone. Naphthoquinones are known as antimicrobial, antifungal, antiviral, antiparasitic, anticancer, and antioxidants (Hara et al, 1997)

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