Abstract
Metabolism of organophosphorus fungicide Hinosan® (O-ethyl S, S-diphenyl phosphorodithioate) by mycelia of P. oryzae, rice blast fungus, was studied using 32P–, 35S– and non-labeled compounds, by ion exchange chromatography, paper chromatography, thin-layer chromatography and gas chromatography, and identifying the metabolites and their derivatives with authentic compounds.The main metabolic pathway is hydrolysis of one P-S linkage followed by the other P-S linkage or ethyl ester linkage and finally yielding phosphoric acid. A part of the fungicide metabolizes to hydroxylated intermediate metabolite, O-ethyl S-p-hydroxyphenyl S-phenyl phosphorodithioate. No significant difference in rate and mode of metabolism was found in this experiment between susceptible and resistant clones against the fungicide.
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