Abstract
Studies on the metabolism of different forms of vitamin B6 in vivo require the use of labeled compounds with high specific radioactivity. Tritium-labeled pyridoxine has therefore been used in several studies. Since tritium is lost in the metabolic conversion of pyridoxine, we have now determined the distribution of tritium in [3H8] pyridoxine prepared by exposure of pyridoxine to tritium gas and compared the metabolism of [3H8] pyridoxine in rats with that of [4,5 – 14C2] pyridoxine. Oxidation of [3H8, 4, 5 – 14C2] pyridoxine with potassium permanganate to 4-pyridoxic acid and to 5-hydroxy-6-methylcinchomeronic acid showed that 22% of the tritium was located in the 4-hydroxymethyl group and 21% in the 5-hydroxymethyl group. The isotope-retention curves of tritium and carbon 14 after administration of [3H8, 4, 5 – 14C2]pyridoxine to rats had approximately the same kinetic parameters, indicating that [3H8] pyridoxine may be used to calculate fractional turnover rates of vitamin B6. Two methods for the calcu...
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