Metabolism of Tritiated Gibberellins A4 and A9 in Norway Spruce, Picea abies (L.) Karst.

Abstract

Shoots of mature grafted propagules of Picea abies (L.) Karst. metabolized [(3)H]gibberellin A(4) (GA(4)) to at least 14 acidic substances, two of which were tentatively identified by gas-liquid radiochromatography as GA(2) (possibly an artifact) and GA(34). [(3)H]GA(9) was converted into a number of metabolites, one of which was chromatographically similar to, but not identical with, GA(4). Metabolism was maximally 61 and 57% over 48 hours for GA(4) and GA(9), respectively, and was correlated with the rate of change (i.e. increase followed by decrease) in endogenous GA-like substances as shoot elongation progressed. Propagules covered with a clear plastic film, a treatment which promotes flowering, metabolized [(3)H]GA(4) more slowly than did control plants in the open. Inasmuch as a GA(4/7) mixture can also promote flowering in P. abies, the retarded metabolism of [(3)H]GA(4) may reflect the manner in which trees under plastic metabolize endogenous GA-like substances. If so, then the stimulating effect of this cultural treatment on flowering may come about through an increased level of endogenous, less polar GA-like substances.