Metabolism of serine in growing rats and chicks at various dietary protein levels.

Abstract

The metabolic fate of the carbon skeleton of L-serine-U-14C has been investigated, in vivo and in vitro, in growing rats and chicks fed the diets with various protein calories percents (PC%) at 410 kcal of metabolizable energy. The incorporation of 14C into body protein at 12 hr after the injection of serine-14C was about 49% of the injected dose in rats fed the 10 or 15 PC% diet, though the value was reduced in rats fed lower and higher protein diets. The 14CO2 production was smaller in rats fed the 10 and 15 PC% diet, and it showed an inverse pattern to that of the 14C incorporation into body protein. Urinary excretion of 14C was higher in rats fed 10 and higher PC% diets, whose growth rate and net body protein retention were maximum. In contrast to the case of rats, the incorporation of 14C into body protein of chicks at 6 hr after the injection was rather reduced in the 15 PC% group. The proportion of 14C excreted as uric acid was remarkably increased above the 10 PC% group, and about 19% of the injected dose was recovered in the 50 PC% group. The catabolic rate of serine in the liver slices of rats and chicks was increased by high protein diets. These results support the concept that the nutritional significance of metabolism of the carbon skeleton of serine in growing rats and chicks is different from each other, especially at high protein diets.