Abstract
Pyruvate-1- 14C, pyruvate-2- 14C and pyruvate-3- 14C, and unlabeled pyruvate in the presence of 14CO 2, were incubated in a bicarbonate buffer with rat epididymal adipose tissue. Incorporation of 14C into CO 2, glycerol and fatty acids of triglycerides, lactate and glycogen were measured and the glycerol and lactate were degraded to yield the relative activities of 14C in each of their carbons. The results are in accord with metabolism via the Embden-Meyerhof Pathway and Krebs Cycle. The dicarboxylic acid shuttle, involving conversion of pyruvate to phosphoenolpyruvate via CO 2 fixation with oxaloacetate as an intermediate, and equilibration with symmetrical dicarboxylic acids of the Krebs Cycle, occurs in adipose tissue and plays a major role in the distribution of the carbons of pyruvate.
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