Abstract
The metabolism of propyl gallate by isolated hepatocytes of the rat and the effect of alcohol on the metabolism were investigated. When the hepatocytes (5×106 cells) were incubated with 10-3M propyl gallate for 1hr, the cells hydrolyzed about 65% of the substrate, and produced large amount of free gallic acid and its conjugate and a small amount of 4-O-methyl gallate. However, the addition of methionine caused an increase in 4-O-methyl gallate formation from propyl gallate.The hepatocytes also produced an unidentified substance in addition to the normal products in the presence of ethanol (5×10-3M). The maximum amount of the unidentified substance in an incubation mixture containing 0.1M ethanol was observed after the incubation for 1hr, and the amount then gradually decreased. The unidentified substance was identified as ethyl gallate by TLC, GC and GC-MS. Ethyl gallate might be produced by the transfer reaction of gallic acid produced by the action of esterase. The ester transfer reaction also occurred with other alcohols such as methanol, iso-butyl alcohol and iso-amyl alcohol, but ethanol was the most effective.
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