Abstract
Small amounts of p-nitrobenzyl alcohol were isolated from chloramphenicol fermentations with Streptomyces sp. 3022a. Cultures supplemented with p-aminobenzoic acid also yielded p-nitrobenzoic acid. Radioactivity from p-aminobenzoic-ring-14C acid was incorporated more efficiently into p-nitrobenzoic acid than into the alcohol; relatively little appeared in chloramphenicol. A large proportion of the radioactivity was found in the mycelium, from which bound forms of labeled p-nitrobenzoic acid and p-nitrobenzyl alcohol were obtained. The results suggest that p-aminobenzoic acid is oxidized to p-nitrobenzoic acid as a covalent complex, and that part of the p-nitrobenzoic acid produced is converted further to p-nitrobenzyl alcohol.
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