Abstract
Metabolism of fatty acid was studied in the isolated rat heart perfused with albumin-bound palmitate-C14. Uptake of fatty acid by the heart was related to the concentration of fatty acid present and the molar ratio of fatty acid to albumin in the perfusion fluid. Disappearance of lipid-bound radioactivity from the perfusion fluid (palmitate-C14 uptake) was consistently greater than disappearance of titratable fatty acid. This was attributed to failure of equilibration of unlabeled "endogenous" fatty acid on albumin with added palmitate-C14 and possibly to release of unlabeled fatty acid from the heart to the perfusion fluid. The principal fates of palmitate-C14 were C14O2 and saponifiable tissue lipid. The maximal rate of recovery of palmitate-C14 as C14O2 was equivalent to 83% of total CO2 production of the isolated perfused heart in 30 min.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
