Abstract
myo-Inositol-2- 14C is not degraded to respiratory 14CO 2 by nephrectomized rats. This and the results of earlier tracer experiments suggest that the kidney is the only organ of importance in inositol catabolism. Rat kidney slices could convert at least 4.7 μmoles of myo-inositol-2- 14C to 14CO 2/gm tissue/hour at inositol concentrations (in the incubation medium) of 12 m m or greater. This is many times the rate obtainable with homogenates but is probably less than the true catabolic rate of the tissue. Cortex, medulla, and the nephron tubules of cortex gave about equal rates, but that of papilla was negligible. Over the range 0.75–9.5 m m (inositol in the medium) the compound was taken up against a concentration gradient. The data indicate that a rat's kidneys can catabolize all of the inositol ingested in an average daily diet. Glucuronate isolated from kidney slices incubated with myo-inositol-2- 14C was the d-isomer. Substantially all the radioactivity was in C-5, as found with purified inositol oxygenase by Charalampous.
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