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Abstract
A pyrophosphatase activity in 15-day embryonic chick lens cells, previously shown to release m 7GMP from 5′terminal mRNA cap, m 7G(5′)pppG(m), has now been examined for its action on a variety of 5′terminal capped sequences of different lengths. The m 7GpppN-pyrophosphatase activity releases m 7GMP from m 7GMP-containing caps m 7GpppN (N = A, Am), and capped sequences (N = GmpCp, AmpApC and AmpApCpApG) but not from m 7GpppN-terminated delta-crystallin mRNA obtained from 15-day lenses. m 7GMP-release from methylated purine caps is inhibited by cleavage product m 7GMP, but is not significantly inhibited by ADP, GDP, PPi, EDTA, poly(A) or by analogous cap molecules FAD and NAD+. m 7GMP, released from caps by the m 7GpppN-pyrophosphatase, can be converted to ribose-5-phosphate and other compounds by the cryoplasmic preparation.
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