Metabolism of glycerol-1,2,3-trimethylsuccinate in rat hepatocytes.

Abstract

The metabolism of glycerol-1,2,3-trimethylsuccinate ester was investigated in rat hepatocytes. The ester displayed a greater nutritional value than D-glucose, as a precursor of either CO2 or glycogen. In terms of 14CO2 production, the value calculated from experiments conducted in the presence of 1.9 mM [U-14C] glycerol-1,2,3-trimethylsuccinate, glycerol-1,2,3-trimethyl[1,4-14C] succinate and glycerol- 1,2,3-trimethyl[2,3-14C] succinate represented about 50 times that found in cells incubated with 1.0 mM D-[U-14C] glucose. For glycogen synthesis, the results found with the ester were approximately 7-8 times higher than those found with the hexose. A further advantage of the ester over D-glucose consisted in the fact that, at increasing concentrations of these nutrients, a maximal metabolic response may be reached at lower levels of glycerol- 1,2,3-trimethylsuccinate than D-glucose. By comparison with previous data obtained in the same experimental model, glycerol-1,2,3-trimethylsuccinate was also found to display a higher nutritional value than the dimethyl ester of succinic acid. It is proposed, therefore, that glycerol-1,2,3-trimethylsuccinate could be used to support ATP generation in cells endangered by an imbalance between the rate of synthesis and hydrolysis of this adenine nucleotide.