Abstract
Leucoplasts and amyloplasts were isolated on Nycodenz gradients from lysates of protoplasts of suspension cultures of soybean ( Glycine max). The labelling of CO 2 and starch was determined after incubating intact and lysed preparations of plastids with 14C-labelled substrates. The substrates were said to have been metabolized by the plastids if the labelling of the product was greater in the intact than in the lysed preparations. Leucoplasts and amyloplasts metabolized both [ 14C]glucose 1-phosphate and [ 14C]glucose 6-phosphate to 14CO 2. In both types of plastid [ 14C]glucose 6-phosphate was the better precursor. Neither substrate was converted to starch by intact leucoplasts. Amyloplasts converted [ 14C]glucose 1-phosphate, but not [ 14C]glucose 6-phosphate, to starch in the presence of exogeneous ATP. It is suggested that in these plastids the reaction catalysed by phosphoglucomutase is not at equilibrium.
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