Metabolism of glucose and glutamate by synaptosomes from mammalian cerebral cortex.

Abstract

Abstract—(1) Synaptosomes incubated in high sodium, low potassium media showed high linear respiration in the presence of glucose which was converted into lactate, aspartate, glutamate, glutamine, alanine and GABA during 1 hr incubation periods.(2) Total conversion of glucose into most of these substrates over the incubation period was similar in synaptosomes and cortex slices. Half the lactate and only a small fraction of the glutamine made by slices was formed by synaptosomes.(3) Pool sizes of amino acids in cortex slices after incubation with glucose were, in general, higher than in synaptosomes, glutamate and glutamine being four‐fold higher in slices.(4) Most of the amino acids made from glucose by synaptosomes were contained within their structure and not lost to the medium.(5) Glutamate was actively metabolized by synaptosomes to aspartate, glutamine, alanine and GABA. The specific radioactivities of the amino acids (except glutamine) after 1 hr incubation, approached that of the glutamate.(6) Pyridoxal phosphate added to the incubation medium increased GABA production from glutamate but not from glucose.