Metabolism of fatty acids in fish. II. Biosynthesis of fatty acids in relation to diet in the carp, Cyprinus carpio Linnaeus 1758

Abstract

Carp, weighing 15–25 g, were maintained in the laboratory on a control diet, or on diets prepared from wheat or corn, and the incorporation of sodium (1− 14C)-acetate into their liver total fatty acids as well as into liver triglycerides and phospholipids was followed. The control diet was characterized by a high concentration of total lipids and linolenic acid, and the other two feeds by a low concentration of total lipids including linolenic acid and high concentration of carbohydrates. The highest rate of fatty acid biosynthesis was observed in fish given the corn diet, and the lowest rate was found in the carp on the control diet. Levels of radioactivity became very high in triglycerides of corn-fed fish. The major products of fatty acid biosynthesis were palmitic and oleic acids, in the corn-fed fish, while in the control fish almost 50% of the total radioactivity was in the long chain polyunsaturated fatty acids. The degree of labeling of oleic and palmitic acids appeared to depend on the level of linolenic acid in the diet. The results suggest that about 1% of linolenic acid in the diet is required to keep lipogenesis at a low level as well as to prevent hyperproduction of oleic acid. Linoleic acid seems to play a subordinate role in regulating lipogenesis in carp liver.