Abstract
THE LOW CONCENTRATION of urinary estrogens in the urine of men and non-pregnant women and the presence of large amounts of inactive phenolic materials has rendered difficult the identification, by isolation, of the nature of the estrogenic compounds present. Dingemanse et al. (1) have isolated estrone from the ketonic fraction of normal male urine. Heard and Hoffman (2) isolated estrone from the weak phenolic ketonic fraction of the urine of a young man after α-estradiol administration and recovered α-estradiol from the weak phenolic non-ketonic fraction of the same urine. We have recently reported the isolation of estriol from the urine of human males injected with estrone (3). The labor and difficulty of such isolation studies should not, however, prevent the determination, particularly in estrogen metabolism studies, by less direct methods of the presumed urinary content of the three native estrogens.
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