Abstract
When day 6 rabbit blastocysts were cultured (3 embryos/mL) in medium 199 containing 3.68 μM estradiol-17β (E 2), 40% of E 2 was metabolized in 24 h, at a rate of 18 pmol/embryo(b)h, yielding 4 major metabolite fractions. Two of them were identified to be estrogen glucosides: 17β-hydroxyestra-1,3,5(lO)-trien-3-yl β-D-glucopyranoside (E 23G) (12 pmol/b/h) and 17-oxoestra-1,3,5(10)-trien-3-yl β-D-glucopyranoside (E 13G) (0.5 pmol/b/h). If the blastocysts were cultured in 3.68 μM E 1 medium, 75% of E 1 was metabolized in 24 h (34.1 pmol/b/h); most of it appears as E 2 (8 pmol/b/h), E 13G (16 pmol/b/h), and E 23G (6 pmol/b/h). Thus, the 17β-hydroxysteroid dehydrogenase activity in the rabbit blastocysts catalyzes mainly in the direction of the E 1→E 2 conversion, with little or no E 2→E 1. This may be responsible in part for the faster metabolism of E 1 than E 2 by the rabbit blastocyst. In comparison with the rat, mouse, and hamster blastocyst, the rabbit embryo shows an additional capability to conjugate large amounts of estrogens into glucosides by steroid glucosyltransferase.
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