Abstract
The metabolism of dicoumarol by rat liver microsomes from untreated and phenobarbital treated rats was studied. Following differential centrifugation of rat liver homogenates only the 104,000 g microsomal fraction supported dicoumarol metabolism. This was found to be strongly dependent on the presence of NADPH or a NADPH-generating system as well as oxygen and it was inhibited by CO. These data indicate that dicoumarol is metabolized by the mixed function oxidase of liver microsomes. The K m and V m for dicoumarol were determined using microsomes from untreated and phenobarbital treated rats. The values of K m were 5·0 × 10 −5 and 3·9 × 10 −5 M, respectively, while the corresponding values of V m were 0·9 and 2·3 nmoles/mg protein/min. The significance of these results is discussed.
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