Abstract
Abstract Animal cells have been considered relatively impermeable to exogenous 3',5'-AMP. The transport of 3',5'-AMP-8-14C from plasma to bile and its metabolism were studied in 28 isolated rat livers perfused for 4 hours. The concentration of radioactivity in plasma, bile, and CO2 was determined at 30-min intervals after 3',5'-AMP-8-14C (3 to 30 µCi) was injected into the portal vein or added to the perfusate. Approximately 13% of the radioactivity was recovered in bile and 3% appeared as 14CO2. Maximal 14CO2 production occurred during the initial 30 min after addition of the tracer dose. 3',5'-AMP and metabolites were isolated from plasma and bile by ascending paper and ion exchange chromatography. Eluates of the paper and ion exchange chromatograms were measured by scintillation counting. The predominant nucleotide during the initial 30-min bile collection was 3',5'-AMP and represented 2.9% of the administered radioactivity. In later bile collections, the radioactivity of this spot markedly decreased, while additional distinct radioactive metabolites developed. These data support the hypothesis that some 3',5'-AMP is capable of penetrating the hepatic cell membrane and indicate that exogenous 3',5'-AMP is rapidly metabolized by the liver.
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