Metabolism of coenzyme A and related nucleotides by liver plasma membranes

Abstract

1. 1. Plasma membranes convert acyl CoA into S-acylpantetheine and several nucleotide residues. The objectives of this investigation were to determine the reaction sequences which are involved in the further metabolism of CoA and of some related nucleotides. 2. 2. The reaction: Acyl-CoA → 4′-phospho- S-acyl pantetheine + 3′,5′-diphosphoadenosine (3′,5′-ADP) was catalyzed by nucleotide pyrophosphatase. 3′,5′-ADP was further metabolized to adenosine and P i with the intermediate formation of 3′-AMP. 3. 3. Liver plasma membrane preparations are characterized by the presence of a variety of phosphoester hydrolases and a labile thioesterase. The properties of these enzymes are described and their possible roles in plasma membranes are discussed.