Abstract
TR-TBT 18d-1 is a syncytiotrophoblast cell line established from placenta of the transgenic rat har- boring temperature-sensitive simian virus 40 large T- antigen (Tg-rat). Previously we have reported high level expressions of drug metabolizing enzymes in the TR-TBT cells. To elucidate the ability of metaboliz- ing xenobiotics in the TR-TBT cells, we measured the metabolism of bisphenol A (BPA) as a model com- pound in the TR-TBT 18d-1 cells. It was found that BPA was metabolized an ds ecreted as a monoglu- curonide conjugate in a manner proportional to the incubation time and the BPA concentration. To elu- cidate the possible effect of green tea consumption on the placental metabolism, we investigated the effect of green tea on the metabolism of BPA in the TR- TBT cells. Green tea inhibited the conjugation reac- tion of BPA at thelow concentration (IC50=5%), how- ever, epigallocathechin gallate (EGCG) showed only weakinhibition, suggestinganotheractive component existence. These results suggest that TR-TBT cells are useful for the model system of rat syncytiotro- phoblasts which act as a barrier to protect fetus from harmful xenobiotics.
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