Abstract
The metabolism of benzo(a)pyrene was studied in the nuclear and microsomal fractions from four rhesus monkey livers. The nuclear fraction was obtained by differential centrifugation, including a step in 2.3 M sucrose. The metabolites from 3H-benzo(a)pyrene were separated by high performance liquid chromatography (HPLC) and the radioactivity was measured. The nuclear fraction catalyzed the formation of 4,5- and 7,8-dihydrodiols and that of 9-OH and 3-OH phenols. The ratio between the nuclear and microsomal rate of formation of the metabolites ranged between 0.02 and 0.03. The nuclear fraction did not catalyze the formation of 9,10-dihydrodiol or quinones at a detectable rate. In the microsomal fraction there was a preferential formation of 4,5-dihydrodiol and 3-OH phenol. Nuclei differ from the microsomes for the lack of formation of quinones.
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