Abstract
α-Ketol of octadecadienoic acid (KODA, 1) has been suggested to play a role in the photoperiod-regulated flowering in Pharbitis nil. The level of 1 in cotyledons is temporarily controlled during short-day conditions. The biosynthesis of 1 is well studied in plants; however, its in vivo conversion is less understood. We have investigated this issue by studying the metabolism of exogenously-applied [U- 13C]- 1, [1- 14C]- 1, and non-labeled 1 in P. nil seedlings by the spectroscopic methods and identified six major metabolites ( 4– 9). We have also found that the enantiomers of 1 are differentially metabolized in P. nil seedlings.
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